Abstract
Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16- and 31 HPV58-positive samples were analysed. E2 integrity was assumed when all fragments covering the E2 gene were amplified with specific polymerase chain reaction primers. Overall, in 59% of the samples, at least one fragment was not amplified in HPV16- (57%) and HPV58-positive samples (61%). Samples from high-grade squamous intraepithelial lesions had the highest frequency of E2 gene disruptions (73%), followed by samples from low-grade squamous intraepithelial lesions (63%) and, finally, samples from invasive cervical cancer (35%). Association between the integrity status of the E2 gene, and lesion grade was assessed by the chi-squared test applied to the combined set of viruses (p = 0.6555) or to populations of the same virus type (HPV58, p = 0.3101; HPV16, p = 0.3024). In conclusion, in this study, no association was found between the presence of E2 gene disruptions and the grade of cervical lesions caused by HPV16 and HPV58.
Highlights
The oncogenic role of human papillomavirus (HPV) has been established through decades of biological and epidemiological studies
Because HPV genome integration is necessary for malignant transformation, the assessment of E2 gene integrity is useful for the determination of the viral genome status
With respect to categorising diagnoses by the histopathological features, 44% of the samples were obtained from low-grade squamous intraepithelial lesions (LSILs), 34% were associated with HSILs and 22% came from women with CC
Summary
The oncogenic role of human papillomavirus (HPV) has been established through decades of biological and epidemiological studies. The aim of this study was to assess E2 gene integrity in HPV16- and HPV58-positive samples as an indicator of the integration of the HPV genome into the host DNA and to determine a possible association of the viral genome status with the grade of cervical lesion.
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