Abstract
The goal of this study was to develop a HPLC method for simultaneously determining bakuchiol, psoralen and angelicin in Fructus Psoraleae (Buguzhi, the fruits of Psoralea corylifolia) and its commercial concentrated products. Extracted samples were analyzed by using a reverse-phase column (Cosmosil 5C18-AR-II) and eluting with a gradient mobile phase consisting of 20% acetonitrile to acetonitrile at a flow rate of 1.0 mL/min with detection at a wavelength of 254 nm. The relative standard deviations of the marker substances on the basis of peak-area ratios in intraday and interday analyses were 0.3-1.5% and 0.2-1.0%, respectively. Bakuchiol, psoralen and angelicin contents were 36.2-71.0, 2.5-13.0 and 2.2-9.2 mg/g for ten raw material samples of Fructus Psoraleae, and 0.6-21.1, 0.6-5.2 and 0.6-5.3 mg/g for eight commercial concentrated products of Fructus Psoraleae, respectively.
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