Abstract
There have been various reports in the literature on the recognition and/or identification of bacterial receptors for host iron-binding molecules. Circumstancial evidence for the role of the putative receptor proteins has been provided by correlations between studies on utilization of ironm and binding of iron-binding molecules, and in a few instances, direct evidence has been provided by genetic means. In this paper we attemp to describe the various approaches that have been utilized for assessing acquisition of iron, binding of ligand and identification of receptor proteins and discuss some of the advantages and limitations of the available approaches. The nature of the question being addressed will determine which of the available approaches is appropriate and may lead to development of alternative methods.
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