Abstract
A method was developed for the analysis of 5-HTP-C 14 and its metabolites in rat liver homogenate. 5-HIAA and neutrals were extracted from the acidified salt-saturated incubation mixture. 5-HIAA was separated from the neutrals by extraction into pH 10 salt-saturated borate buffer. After making the residual incubation mixture alkaline, serotonin was separated by acetylation and extraction into isopropyl acetate. The residual aqueous solution was acidified and the acetylated 5-HTP extracted into isopropyl acetate. The method was used to study the alteration of dl-5-HTP-C 14 metabolism in rat liver homogenate by NAD and NADH. In the unfortified incubation mixture, 5-hydroxyindoleacetaldehyde was the major product; in the NAD incubation mixture, 5-HIAA was the major product; in the NADH incubation mixture, 5-hydroxytryptophol was the major metabolite.
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