Abstract
A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreas- ing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles.
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