Analisis Keragaman Genetik 161 Aksesi Mangga Indonesia Menggunakan Marka Mikrosatelit

Abstract

<p>Mango is one of the five<br />important fruit crops in the world. Microsatellite markers can<br />be used to analyze genetic diversity among mango<br />accessions. The purpose of this research was to determine<br />the relationship among mango germplasm collection using<br />microsatellite markers. A total of 161 mango accessions<br />originated from Indonesian Tropical Fruit Research Institute<br />(Cukurgondang Field Station), Pasuruan, East Java, were<br />used in this research. Twenty-six microsatellite markers<br />were used to genotype each accession. Genotyping was<br />conducted using Beckman Coulter® CEQ™ 8000 machine.<br />Genetic relationship among accecions was analyzed using<br />the Unweighted Pair Group Method with Arithmetic Mean<br />(UPGMA) method, followed by bootstrap analysis. The result<br />showed that high allele variation (15-75 alleles) was<br />observed among mango accesions tested, with an average<br />allele number of 38.69. The average of Polymophism<br />Information Content (PIC) value was 0.548 (0.021-0.949).<br />Fifteen microsatellite markers showed PIC value >0.5<br />indicated that these markers were suitable for mango<br />diversity studies. Cluster analysis divided the mango<br />collections into two groups. Group I consisted of 95<br />accessions, and group II consisted of 66 accessions. Ninety<br />Indonesian indigenous mangos (84.11% of Indonesian<br />mango accessions) could be separated from the introduced<br />accessions.</p>