Abstract
Elephant dung (ED) and Napier grass (NP) are used as waste-to-energy resources that help in waste management and reduce greenhouse gas emissions, in accordance with Sustainable Development Goal 13 (SDG 13). In this study, anaerobic co-digestion of ED and NP (with and without biological pretreatment) was conducted under various mixing ratios. The biological pretreatment of NP was performed using the microbial activator super LDD1 (mixed culture microbial sludge) enzyme before further biogas production. The results confirmed that LDD1 enzymatic microbes increased methane production capacity by 1.95 times compared to untreated NP. Moreover, the data revealed that a NP/ED ratio of 1:1 with a 14-day pretreatment duration yielded the highest cumulative methane production of 234.8 ± 5.9 mL-CH4/g-VS, a 99.2% increase. The synergistic index results showed that substrate pretreatment had the most significant effect on methane yield, with the maximum synergistic index equaling 1.53. The Gompertz model outperformed the Logistic model in determining the reaction's kinetic parameters, with R2 > 0.995.
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