Abstract
Vicenin-2 (VIC) and lychnopholic acid (LA) are present in large amounts in hydroalcoholic extract from leaves of Lychnophora salicifolia, commonly known in the Brazilian Cerrado as arnicao, a plant applied as topic anti-inflammatory and as a flavoring agent for the traditional spirit (cachaca). A new ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for quantification of LA and VIC in plasma samples in order to evaluate their pharmacokinetic profiles in rats. The method was linear in the range of 10-2500 ng mL-1, acceptable precision accuracies were obtained and no significant matrix effect was observed. The validated method was successfully applied to pharmacokinetic study. LA presented very low t1/2 (half-time), high Cl (serum clearance) and Vd (volume of distribution), and for VIC, the t1/2 value was slightly larger, but still rather low, whereas Cl and Vd values were slightly lower, indicating that both substances are instantaneous and well distributed in the body, and quickly eliminated.
Highlights
The Lychnophora genus has been the focus of our research group (Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS, FFCLRP-USP)) that for many years has been studying several species with many applications, like in traditional medicine as topical analgesic and anti-inflammatory, and as flavoring for the Brazilian traditional spirit cachaça.[1]
The analytical methodology was validated in accordance with current Brazilian and international guidelines and successfully applied for the analysis of the samples from our pharmacokinetic study.[15,16]
The results showed that vitexin was rapidly and widespreadly distributed throughout the whole body after administration and the oral bioavailability was 3.91%
Summary
The Lychnophora genus has been the focus of our research group (Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS, FFCLRP-USP)) that for many years has been studying several species with many applications, like in traditional medicine as topical analgesic and anti-inflammatory, and as flavoring for the Brazilian traditional spirit cachaça.[1]. VIC and LA are the major constituents of hydroalcoholic extracts prepared from leaves of L. salicifolia and can be isolated, as described before.[14] Considering that, the aim of this study was to develop and validate a method using UPLC coupled with tandem mass spectrometry (UPLC-MS/MS) to quantify VIC and LA in rat plasma samples after single intravenous dose.
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