Abstract
Ascaris suum, an intestinal parasite of swine, has a complex body wall, consisting of nine distinct layers when observed by transmission electron microscopy. The outermost layer of the cortex is a trilaminar region, the epicuticle, that is similar in appearance to plasma membrane. Parasitic nematodes interact with their hosts through this layer. The structure of the epicuticle is being studied with transmission and scanning electron microscopes.Parasites were obtained from an abattoir at Waterloo, Iowa, and maintained in Harpur's saline medium for up to two days. Cuticles were separated by microdissection and washed in 0.1M phosphate buffer, pH 7.2. Trump's universal fixative (4% Formaldehyde: 1% Glutaraldehyde in phosphate buffer, pH 7.2 ) was used for 1 h at room temperature for primary fixation. Surface charge of epicuticle was studied by incubating cuticle with cationized ferritin (Polysciences) at a concentration of lmg/ml in 0.1M phosphate buffer, pH 7.2, for 30 minutes at room temperature.
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