Abstract
Purpose: Understanding the distribution and interaction of the Traditional Chinese Medicines (TCMs) is an integral source of herbal drug discovery. An optimized radio-labeled method was explored that could conduct in situ biodistribution studies in animals. We evaluated the feasibility of the method and applied glycyrrhetinic acid and platycodon (PG) polysaccharides as models.Procedures: [18F]-GA is a novel radiotracer which was performed positron emission tomography (PET) studies to assay the biodistribution of GA in mice. In addition, PG polysaccharide was used to intervene the biodistribution and dosimetry of GA. Scanning data were analyzed with professional software.Results: Record the time-activity curves for all organs then use the normalization method to calculate the area under the curve as a dosimetry for each organ. Moreover, the addition of PG polysaccharides can significantly improve the dosimetry of GA in the lungs, and its effect was related to the administration time.Conclusion: MicroPET imaging opens up a new avenue for the application of drug interactions between the TCMs.
Highlights
Traditional Chinese Medicine (TCM) has many compounds with complex structures and multiple biological activities (Amirkia and Heinrich, 2015)
The results showed that the combination of glycyrrhetic acid (GA) and Platycodon grandifloras (PG) polysaccharides could effectively change the distribution of drugs
Our data demonstrated that MicroPET could be used to study the biodistribution of natural products
Summary
Traditional Chinese Medicine (TCM) has many compounds with complex structures and multiple biological activities (Amirkia and Heinrich, 2015). It is widely recognized that tissue distribution of natural products is a huge task, many successful examples of molecules target confirmation have been reported, such as blood concentration monitoring, liquid chromatography-mass spectrometry (LC-MS), and so on (Takahashi et al, 2010). These technology were conventional methods for drug tissue distribution (Dixon et al, 2007); the most serious issue preventing such an approach is the difficulty in a large number of animals and repeated experiments, time-consuming and laborious.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
