Abstract
India is bestowed with a rich wealth of medicinal plants due to its diverse agro climatic and regional topography but indiscriminate collection from the wild has brought much of this biodiversity to the brink of extinction. The present research was aimed to introduce a commercially viable rare medicinal plant, Colchicum luteum from the semi-temperate, high altitude region of our country to the tropical agroclimatic zone of lower Indian Gangetic plains in view of producing the raw material of the species at higher rate than that obtained by natural propagation. Colchicine, the commercially important secondary metabolite of the plant is used in conventional crop breeding, in preparing anti-gout drugs and in anti-cancer research. A high multiplication rate of C. luteum (12 shoots on average/explant) was achieved in vitro by culturing small segments of bulbs in MS medium1 supplemented stepwise with 8.88µm BAP for shooting and IAA (5.71µm) or IBA (4.92µm) for rooting and bulblet formation. The hardened plants survived ex-vitro by 99%. Colchicine of 1 year old bulbs was estimated by TLC and HPLC followed by UV-vis spectral analysis. Genetic fidelity of the in vitro-raised plants was checked using a genetic marker. The finding of our study ensures feasibility of cultivation of this important plant in the proposed agro climatic region and may provide scope to generate a source of income, especially for the underprivileged section of the rural community.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
