Abstract
BackgroundPAX2 is a transcription factor with an important role in embryogenic development. However, PAX2 expression was frequently identified in neoplasia responsible for the growth and survival of cancer cells. Due to alternative splicing of exon 6, exon 10 and exon 12 four isoforms of PAX2 are described so far.MethodsThe expression of an intron 9 containing PAX2 splice variant was analyzed in neoplastic B cell and solid tumor cell lines as well as in primary tumor samples by quantitative RT-PCR. PAX2 proteins were detected by Western Blot in a subset of cell lines.ResultsAll 14 lymphoma cell lines expressed an undescribed PAX2 splice variant containing the entire intron 9 sequence and the exon 10 sequence. This splice variant could also be detected in 35 solid tumor cell lines, in leukemia and lymphoma as well as in colon carcinoma and melanoma patient samples and in blood samples of healthy donors. Expression of this new splice variant on protein level was verified by Western Blot analysis.ConclusionWe discovered a previously undescribed intron 9 and exon 10 containing splice variant of PAX2 in B-cell neoplasia and in solid tumors on mRNA and protein level.
Highlights
PAX2 is a transcription factor with an important role in embryogenic development
Cells and Reagents 14 lymphoma cell lines (AMO-1, DG75, EHEB, KARPAS422, KM-H2, HDLM-2, L540, RAJI, SU-DHL-4, SUP-M2, U698, U937, U266, BONNA-12) and 35 solid tumor cell lines (4 thyroid cancer cell lines: 8505C, CGTHW-1, BCPAP, TT260Co2; 7 renal cell carcinoma cell lines: A706; Caki1; ACHN; A498; SN12; CC5; Caki2; 8 melanoma cell lines: SKMel23, Mel10, Mel16, Mel-HO, SKMel24, SKMel5, Mel28, 624.28; 8 colon carcinoma cell lines: SW620, HCT116, Cx94, CaCo2, Colo320, SW480, Colo205, HBL 100, 5 breast cancer cell lines: Mx1, T47D, MCF7, MDA-MB436, BT474; 3 lung carcinoma cell lines: Column6, A427, DMS79) All human cell lines were purchased from DSMZ (Braunschweig, Germany) and CLS (Eppelheim, Germany)
Detection of a new splice variant in tumor cell lines and tissue by RT-PCR RT-PCR analysis of the PAX2 transcript in 14 lymphoma cell lines using a forward primer lying in exon 9 and a reverse primer lying in exon 10 showed different PCR products on gel electrophoresis: All lymphoma cell lines showed a band of 339 bp of varying intensity
Summary
PAX2 is a transcription factor with an important role in embryogenic development. PAX2 expression was frequently identified in neoplasia responsible for the growth and survival of cancer cells. The PAX2 gene is located on the short arm of chromosome 10, locus 24–25 [4] and encodes a transcription factor that has a critical role in the development of the urogenital tract, the eyes and ears, and the CNS [5]. It belongs to the subgroup 2, characterized by the octapeptide sequence and a truncated homeodomaine [6] and is composed of 12 exons spanning approximately 86 kb [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
