Abstract
Aim:Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users.Results:We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid media. We describe a proof-of-concept for the integrated isolation of bacteria, cell lysis with optional DNA extraction, DNA amplification and detection in two different reactions, loop-mediated isothermal amplification and PCR, on a single microfluidic platform.Conclusion:The platform enables the transition from large sample volume to microfluidic format. The design and open interface enable its versatile application for various nucleic acid-based assays, from simple to complex setups.
Highlights
We present a prototype of a table-top instrument, a microfluidic cartridge and assay protocols for DNA-based detection of bacteria on the platform
Model buffer samples spiked with E.coli and Salmonella enteritidis were used
We demonstrate the assays as proof-of-concept and did not evaluate their quantitative characteristics at this moment
Summary
Processing of the samples in molecular diagnostics is complex and labor-intensive. We aim to perform the investigation of the LOD after these improvements and hope to satisfy the interest of the readership in a follow-up article.
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