Abstract
An in vitro method for characterising the degradation of glass fibres in an intramacrophage-type environment is reported. Human blood monocytes provide the phagocytically active cultures and a “semi-dynamic cell replenishment method”, whereby cultures are replenished every three weeks with monocytes from the same donor, has enabled exposure times exceeding 26 weeks to be achieved. Tests indicated an affinity of monocytes for glass fibre, and superoxide activity in freshly isolated cells was measured and found to be comparable with that for alveolar macrophages.
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