Abstract
In the present study, an assay using polymerase chain reaction (PCR) was developed for detecting Enterobacter sakazakii in infant formula. Based on solvent extraction technology, FTA filter was used to extract E.sakazakii DNA from artificially contaminated infant formula. The FTA paper coat was able to efficiently remove inhibitors that could affect the PCR reaction. Primers targeting the 16S rRNA gene were used to amplify a 149 bp DNA fragment, which was confirmed by DNA sequencing. Experiments to determine the sensitivity of the PCR indicated that it could detect as few as 7times102 CFU/ml of E. sakazakii bacteria in infant formula directly and 7times100 CFU/ml after a 4-h enrichment step. This novel FTA-PCR assay allows for detection of E.sakazakii in infant formula in < 6 h, this is a substantial improvement over the conventional PCR with enrichment method which requires 7 days. Thus, PCR amplification using FTA filters provides a faster and more sensitive method of E.sakazakii detection than the standard cultivation method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
