Abstract
A simple method for preparing mitotic chromosomes from adult mouse liver is described. The procedure involves the accumulation of metaphases in regenerating liver resulting from CCI4treatment, followed by Trypsin perfusion, hypotonic pretreatment and fixation of cells, and flame-drying of slides to spread chromosomes. Approximately 2 × 106intact liver cells can be obtained from a single mouse liver – enough to prepare 50 slides. A peak mitotic activity, with more than 1% of cells in mitosis, was observed 72 hr after subcutaneous injection of 0.1 ml of 45% CCI4per animal. The distribution of diploid, tetraploid, and octaploid cells in mitosis was about 86, 11, and 3%, respectively. The abundant number of analyzable metaphases in such preparations makes this method valuable for cytogenetic analyses of normally non-proliferating tissue from adult laboratory mammals.
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