Abstract
An improved methodology is described for the separation of yolk IgG into subpopulations using immobilized metal ion (Fe 3+) affinity chromatography. The yolk IgG was first extracted using a prechilled, pre-acidified method. After extraction, the yolk IgG was then fractionated using an Fe 3+ column. Using an ascending pH gradient, four IgG containing peaks were well resolved based upon the elution pH, specific activity and the relative avidity index.
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