Abstract
ABSTRACT A modification of an androgen assay employing chick comb growth as its end-point has been described. Reduction in the daily inunction volume of the androgen oil vehicle from 0.05 ml to 0.005 ml and its delivery to the comb with a microsyringe resulted in increased assay sensitivity and precision. Statistical analyses of testosterone dose response curves indicate that 6.2 and 6.6 times as much androgen is required when the larger vehicle volume is used to elicit identical comb growth response compared with that of the smaller volume. The index of precision (λ) for a four dose level assay was 0.336 for the larger volume and 0.190 for the smaller vehicle volume.
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