Abstract
An extracellular substance that had single-electron oxidation activity was isolated from wood-containing cultures of the white-rot basidiomycete Phanerochaete chrysosporium. The substance was partially purified by acetone precipitation, gel filtration chromatography on Sephadex G-50 and G-25, and DEAE Affi-Gel Blue gel chromatography. It contained about 18% protein, 20% neutral carbohydrate and 0.28% Fe(II) by weight. This substance was low molecular weight and could catalyze redox reactions between an electron donor, such as NADH, and O 2 to produce H 2 O 2 and to reduce H 2 O 2 to OH. It reduced Fe(III) to Fe(II) and strongly absorbed Fe(II). This extracellular substance seems to be involved in the initial degradation of cellulose and lignin in wood by the fungus.
Published Version
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