Abstract
Paper chromatograms on which different concentrations of various chromogens and fluorogens were separated were assessed in the reflectance and transmittance modes of operation in a double beam, flying spot, ratio forming prototype scanning photodensitometer. Linear calibration curves were obtained in most instances with the lower level of detection in the case of colored substances being about 6.2 × 10 −11 moles and in the case of fluorescent substances being about 5.0 × 10 −12 moles. The scanning device exhibited a stability and reproducibility as high as ±0.7% (fluorescence0 and ±1.3% (absorption-transmission) when moderate quantities (0.5 μg) of fluorogens and chromogens were separated. These values decreased to ±6.4% (fluorescence) and ±8.5% (absorption-transmission) respectively when much smaller quantities (25–100 ng) were separated. The assessment of separated chromogens by reflectance appeared in the main to be less satisfactory than the transmittance procedure.
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