Abstract
In the recent decade, GO has emerged as an amazing 2D nanomaterial for developing DNA-based biosensors due to its fluorescence quenching properties, whereas similar research based on rGO was reported rarely. Herein, a novel multi-pyrene functionalized G-rich DNA probe based on the screened rGO showed much higher fluorescence quenching efficiency and excimer emission than that of universal GO. Different from the universal thrombin detection of the G4-forming aptamer-TBA(GGTTGGTGTGGTTGG), the original telomeric sequence is used in this study. The excimer emission “ON-OFF” switch amplified the response of thrombin detection is as low as 50 units. Furthermore, for four pyrene moieties that are sited in a crowded steric circumstance, the melting temperature (Tm) values and molecular dynamics simulations showed a positive effect on duplex G-quadruplex or GDNA·cDNA stability, without disturbing its helix structure.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
