Abstract
A probe with an isothiocyanate group was synthesized and evaluated for its H2S sensing ability. Upon addition of H2S, the probe exhibited ratiometric properties during absorption with a red-shift. The probe exhibited fluorescent off–on responses towards H2S via the ESIPT process, due to the conversion of isocyanate into amine. UV–vis, fluorescence, and 1H NMR spectroscopic analyses were performed to investigate the sensing mechanism. The probe has a large Stokes shift, short response time, and low detection limit. It can be used to estimate H2S levels within the range of 0–36 nM. The practical applicability of the probe was demonstrated using water samples and living cells.
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