Abstract
An enzymatic flow analysis approach, based on the Griess–Ilosvay reaction, was developed for the evaluation of nitrite and nitrate, aiming at an environmentally benign alternative reduction method using nitrate reductase and the development of an in-line dilution procedure for the analysis of water samples, with a wide range of nitrate concentrations. Nitrate concentrations up to 250 mg/L can be analysed directly in the automatic system, by changing the aspirated sample volume or by applying the in-line dilution procedure provided by the automatic system. Under the optimum conditions, the calibration curves were linear up to 50 mg/L of nitrate and 3 mg/L of nitrite, with the detection limits being 0.73 mg/L and 0.03 mg/L, respectively. Nitrate and nitrite were determined in different water types by the flow method with rsd<5%. Comparison with the reference methods showed no statistically significant differences for a 95% confidence level.
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