Abstract
The aim of the present work was the development of an ELIME-array to achieve simple and rapid detection of AFB 1 in corn samples. The system is based on an indirect competitive ELISA format using magnetic beads as immobilisation support and eight magnetised screen-printed electrodes as electrochemical transducers. After an optimisation study, a corn sample treatment, employing an extraction in acetonitrile/water followed by a clean-up step and solvent evaporation, was selected. For the construction of the calibration curve, which was used to evaluate both evaluation of the matrix effect on the performances of the ELIME-array and for the analysis of Certified Reference Materials (CRMs), standard solutions of AFB 1 were added to blank dried corn extracts reconstituted in PBS. The detection limit and the sensitivity of the assay were calculated to be 0.6 ng mL −1 and 1.5 ng mL −1, respectively. Precision (11–26%) and recovery (95–114%) data of the ELIME-array, determined by analysing four CRMs, have shown that the proposed system appears suitable as a screening tool for the analysis of AFB 1 in corn samples.
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