Abstract
Alabel-free electrochemical aptasensor is reportedfor sensitive detection of the 6-kDa early secreted antigenic target (ESAT-6). For the first time, the bimetallic organic framework (b-MOF) of Zr-MOF-on-Ce-MOF was decorated with nitrogen-doped graphene (NG) and applied as the matrix for electroactive toluidine blue (Tb) to form the NG@Zr-MOF-on-Ce-MOF@Tb nanohybrid. Theprepared nanohybrid with excellent hydrophilicity, dispersibility, and large specific surface exhibited significant electrochemical response. Thisnanohybrid could be directly used for anchoring ESAT-6 binding aptamers (EBA) through the interaction between the 5'-phosphate group (PO43-) of EBA and Zr4+ of Zr-MOF. The signal response before and after incubating the ESAT-6 antigen has beenevaluated by cyclic voltammetry at a scan rate of 100mVs-1 from - 0.7 to 0.3V (vs. SCE). Under optimal conditions, the proposed aptasensor displayed a wide linear range from 100fgmL-1 to 10ngmL-1 with a limit of detection (LOD) of12fgmL-1. The developed method showed good reproducibility with a relative standard deviation (RSD) of 2.27%. Theaptasensor showed favorable results in the analysis of the real samples. With these merits, the aptasensor has exceptional potential as a diagnostic tool for tuberculosis in clinical practice.
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