Abstract

Abstract Cellular assays for suspension cells, especially immune cells, provide investigators an opportunity to interrogate the biology of cellular function of immune cells or immunotherapeutic drugs. Despite significant technical advances made during the last few decades, working with suspension cells, such as primary blood cells, PBMCs (peripheral blood mononuclear cells), leukemia, myeloma, or lymphoma cells, current cellular assay approaches still suffer from limitations. For example, significant cell loss can occur during assay process. This loss can be further exacerbated when multi-step staining procedures are required. We applied DropArray plates, which employ surface tension to retain cells on the plate surface, for our suspension cellular assays. For a variety of suspension cells, including PBMCs, AML cells, or B cells, efficient retention (50% to 80%) is achieved during multiple washes. Reproducibility and quality of the experimental results are improved with DropArray plates. Furthermore, specific cytotoxic action of therapeutic antibodies on tumor cells can be also observed in real time. In summary, this system presented an efficient approach for phenotypic study of suspension cells, especially rare population of cells.

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