Abstract
Magnolia sirindhorniae Noot. & Chalermglin is an endangered species with high ornamental and commercial value that needs to be urgently protected and judiciously commercialized. In this study, a protocol for efficient regeneration of this species is standardized. The lateral buds of the M. sirindhorniae plant were used as an explant. Half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 2.0 mg/L gibberellic acid (GA3) was found to be the optimal medium for shoot induction. The maximum shoot multiplication rate (310%) was obtained on Douglas-fir cotyledon revised medium (DCR) fortified with 0.2 mg/L BA, 0.01 mg/L NAA, and additives. The half-strength DCR medium supplemented with 0.5 mg/L NAA and 0.5 mg/L indole-3-butyric acid (IBA) supported the maximum rate (85.0%) of in vitro root induction. After a simple acclimatization process, the survival rate of plantlets in a substrate mixture of sterile perlite and peat soil (1:3; v/v) was 90.2%. DNA markers were used for assessment of genetic uniformity, confirming the genetic uniformity and stability of regenerated plants of M. sirindhorniae. Thus, the described protocol can safely be applied for large scale propagation of this imperative plant.
Highlights
Magnolias (Magnoliaceae) have long been popular and widely cultivated as ornamental plants, shrubs, and trees
Considering growth and induction rate, half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L BA, 0.1 mg/L naphthaleneacetic acid (NAA), and 1.0 mg/L GA3 turned out to be a better medium for in vitro induction, as it supported maximum shoot bud induction (Fig. 1b)
Direct multiple shoot induction suppresses the risk of genetic instability
Summary
Magnolias (Magnoliaceae) have long been popular and widely cultivated as ornamental plants, shrubs, and trees. Due to the decline of its habitat, it was classified as ‘Endangered’ on the IUCN Red List[7] It is difficult for M. sirindhorniae to reproduce by seeds due to the low percentage of fruit setting. No studies have reported a protocol for efficient regeneration of this important rare species. We explored an efficient protocol for in vitro plant regeneration via shoot induction of M. sirindhorniae for the mass propagation of this precious magnolia plant. The genetic uniformity assessment of regenerated plants is of great importance. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) have successfully used for assessing the genetic fidelity of regenerated plantlets in many plant species[16,17,18]. This research will be of great help to preserve this important species
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