Abstract
The E2F transcription factors play important roles in the regulation of gene expression at the G1/S transition in plants. Here, we show that the rice proliferating cell nuclear antigen (PCNA) promoter is activated by transient expression of tobacco NtE2F and Arabidopsis AtDPa in tobacco cells. This transcriptional activation is repressed by co-transfection with a plasmid encoding the tobacco Rb-related protein (NtRBR1), whereas further co-expression of cyclin D overcomes this repression. Importantly, the rice PCNA promoter is activated when cells are transfected with cyclin D alone, and this activation is enhanced by co-transfection with plasmids encoding NtE2F and AtDPa. These results suggest that the effect of cyclin D expression is mediated not only by its associated kinase, which allows it to phosphorylate NtRBR1 thereby releasing the NtE2F/NtDP complex to activate transcription, but also by a mechanism which does not involve transfected NtRBR1.
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