Abstract
A type II restriction endonuclease (SuaI) has been isolated from the thermoacidophilic archaebacterium Sulfolobusacidocaldarius. The enzyme is an isoschizomer of BspRI. It does not cut S. acidocaldarius DNA, as the recognition sequence GGCC in this DNA contains modified nucleotide(s). The enzyme is most active at 60–70°C and is highly thermostable.
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