Abstract
No single analytical technique will provide all the information necessary for the interpretation of a complex biological system. The mineralisation of bone is no exception, especially in a pathological situation. Brittle Bone disease, a genetically and biochemically heterogeneous group of disorders is characterised by the ease and frequency of bone fracture. Although Type I procollagen mutations have been detected, few reports exist of the mineral and its relationship with the altered collagen.X-Ray Microanalysis (XRMA), X-Ray Powder Diffraction (XRPD), Fourier Transform Infra Red spectroscopy (FTIR) and31p Nuclear Magnetic Resonance spectroscopy (31P NMR) were used to study the composition, structure and resonance of the calcium-phosphate (Ca-P) in bone. Specimens of bone were fixed in 2.5% gluteraldehyde solution (18 hours) at 40C. The specimens were cut into small pieces (1-3mm3) during the first hour of fixation, followed by secondary fixation in 1% osmium tetroxide. The tissue was dehydrated through a graded series of ethanols before vacuum infiltration and embedding in Spurr's resin for 18 hours at 600C.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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