An analysis of the deformed erythrocytes correlated to varied dose of nanoparticles emitted by diesel engine bus

Abstract

This study was aimed to investigate the morphological changes of the mice’s erythrocytes corresponded to the bus exhaust nanoparticle exposures. Male mice were used as the experimental animals. The exhaust emissions emitted by bus samples B1, B2, and B3 were filtered using a nanoparticulate filtering system consisting of an N95 mask and a sucking pump, and the concentration was measured using a TSI P-Trak Ultrafine Particle Counter. In order to the erythrocyte deformation, we used the unexposed and the exposed mice to the bus exhaust emission with the varied particle concentration of Ld - low dose, Md - medium dose, and Hd - high dose as long as 100 seconds per day in eight consecutive days. Then, all mice were sacrificed for the erythrocytes analysis. We found two deformation types that were most highly increased in the erythrocytes such as: helmet-shaped cells and teardrop-shaped cells. The deformation percentages were in the range of 27% to 47%, depending on the nanoparticle concentration dose. Ld had the lowest deformation percentages: 28% for B1, 34% for B2, and 44% for B3. The highest deformation was found in Hd, with the values of 30%, 37%, and 47%, respectively for B1, B2, and B3.