Abstract
Methods First, a novel T cell epitope from human heat-shock protein 60 (Hsp60), an autoantigen involved in the pathogenesis of JIA and other autoimmune diseases, was identified by bioinformatics tools and an APL was design starting from this epitope. We evaluated the therapeutic effect of this peptide in an adjuvant-induced arthritis (AA) rat model. Clinical score, TNFa levels and histopathology were monitored. Also the potentialities of the APL for inducing regulatory T cells were evaluated in ex vivo assays using peripheral blood mononuclear cells (PBMC) from JIA patients. Results The APL efficiently inhibited the course of AA in rat, with significant reduction of the clinical and histopathogy score. This effect was associated with a decrease of TNFa levels in spleen. Finally, stimulation of PBMCs from JIA patients by the APL increases the proportions of the CD4+CD25 FoxP3+ Treg cells.
Highlights
Induction of immune tolerance as therapeutic approach for autoimmune diseases constitutes a current research focal point
The Altered Peptide Ligand (APL) efficiently inhibited the course of adjuvant-induced arthritis (AA) in rat, with significant reduction of the clinical and histopathogy score
Stimulation of peripheral blood mononuclear cells (PBMC) from Juvenile idiopathic Arthritis (JIA) patients by the APL increases the proportions of the CD4+CD25high FoxP3+ Treg cells
Summary
Dolores Cantera1*, Noraylis Lorenzo, Ariana Barberá, Amarys Alonso, Yamile Heredia, Elsy Chall, Lourdes Franco, Ramón G Padrón, Maria C Domínguez. From 18th Pediatric Rheumatology European Society (PReS) Congress Bruges, Belgium. From 18th Pediatric Rheumatology European Society (PReS) Congress Bruges, Belgium. 14-18 September 2011
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