Amyloid β Detection with Integrative Chip for Alzheimer Desease

Abstract

To enhance sensitivity and detection limit, may research have been reported both with new materials and hybridization of two different devices/functions. Recently, due to high preconcentrator factor and simple method, the interest of ion concentration polarization (ICP) for sensor has been increasingly recognized. Here, we report ion concentration polarization based preconcentrator integrated with electrical biosensor to overcome low affinity of low concentration. We achieve approximately 25-fold concentrated sample on IMEs sensor with ICP preconcentrator. Utilizing ICP preconcentrator integrated IMEs sensor, we are able to detect below 22.15 fM Aβ with 3.2-fold higher sensitivity of impedance change. Moreover, detection limit of Aβ has also improved under concentration of 2.215 fM with ICP preconcentrator integrated IMEs sensor. From these results show our newly proposed ICP preconcentrator integrated IMEs sensor was better than conventional method for detection and quantification of low Aβ level and affinity. Also, a unifying technique between preconcentration and sensor platform is provide new opportunities with analytical sensitivity and detection limit within a few minutes.