Abstract
Objective To determine a method for amplification of cytochrome C oxidase subunit I(CO I) gene of Brandt's vole. Methods The Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia, and DNA was extracted from liver tissue. CO I gene was amplified by nested PCR and sequenced afterwards. Results A band of 657 bp and 1 132 bp was amplified by internal and external PCR primers, respectively, which were consistent with expected sizes. A total of 12 segments of Brandt's Vole CO I gene sequences were amplified by PCR and verified by sequencing. The sequence number was KF182196 - KF182207 in GenBank. After gene sequence alignment of the 12 CO I gene sequences, it was found that the similarity was 100%, and no base mutation. Conclusion CO I gene of Brandt's Vole could be amplified by nested PCR without pseudo gene. Key words: Nested PCR; Brandt's Vole; Cytochrome C oxidase subunit I gene
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