Abstract
A nanocomposite prepared from reduced graphene oxide (rGO) and silver nanoparticles (AgNPs) is used in an electrochemical aptasensor for the sensitive and selective determination of the antibiotic chloramphenicol (CAP). The nanocomposite was obtained by electrostatic assembly of AgNPs on the surface of polyelectrolyte-functionalized rGO and then used to modify a glassy carbon electrode. The biosensor is then obtained by immobilizing the aptamer against CAP. When incubated with solutions of CAP, the sensor surface is loaded with CAP due to aptamer recognition. The captured CAP can be electrochemically reduced to yield a current that is strongly enhanced as a result of the excellent electrocatalysis property of the graphene/AgNP-nanocomposite. Under optimum conditions, the calibration plot is linear in the 0.01 to 35 μM concentration range, with a 2 nM detection limit (at 3σ). The sensor is reproducible, stable, selective over homologous interferents, and performs excellently when analyzing CAP in milk samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
