Abstract
In this work the use of Polyurethane (PU)-Chitosan(CH) scaffolds prepared by thermal induced phase separation (TIPS) for osteoblast proliferation and bone mineralization is described. Primary rat calvaria osteoblasts were seeded in the scaffolds and it was shown that supported cell adhesion and growth. The behavior osteoblast cells growing in the scaffold in function of the different ratio of PU and CH is presented. The results showed that TIPS is an appropriate technique for the production of PU-CH scaffolds with high potential for application as cell scaffolds in bone tissue engineering.
Highlights
Tissue engineering combines cell biology, materials chemistry, and processing to recreate viable tissue that restore and maintain the function of the body
90/10 and 50/50 composites duplicate them adherence, opposed to the fast increase in cell number observed during the first hour, chitosan increases only from 30% to 35% and the composite 70/30 from 17% to 22%, respectively
Chitosan content in the composite plays an essential role in osteoblast adhesion; since chitosan has a higher hydrophilicity than polyurethane that deals with a more favorable topography and chemistry or surface energy for pure osteoblast adhesion, chitosan has a higher adherence than the PU and composites
Summary
Tissue engineering combines cell biology, materials chemistry, and processing to recreate viable tissue that restore and maintain the function of the body. The scaffold fabrication technique needs to be developed appropriately to manufacture the scaffold with the desired physical-chemical and mechanical properties. Factors such as porosity and pore size are very important for vascularization and the tissue in growth but they are difficult to control precisely during the process [2]. The PU has been utilized for the development of several kinds of materials and devices for biomedical applications, the preparation process and its velocity of degradation can be controlled and the physical and mechanical properties can be adjusted. In we present the evaluation for application for bone tissue engineering by means of seeding osteoblast rat calvaria cells into the scaffold; the results in cell proliferation and growth as well its mineralization suggest that these scaffolds are potentially appropriate for bone tissue engineering
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