Abstract
200 μm whole body sections of pre-treated laboratory animals were inoculated with vitamin- or aminoacid-dependent micro-organisms and incubated. Live micro-organisms are revealed by tetrazolium salts and furnish vitamin or aminoacid distribution maps. In particular methionine, cyanocobalamin and thiamine distribution is studied; to verify that maps record only these agents, specific antimetabolites were used (Friedrich's S-102 and α-methyl-methionine) 1.
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