Abstract
The aims of this research is to determine pathotype according to composition amino acid cleavage site of F protein, and epitope prediction of F protein from Surabaya isolate. Samples were cloacal swabs of 28 backyard chickens from five wet markets in Surabaya, 9 ND positive isolates from Laboratory of Virology and Immunology Department Microbiology Airlangga University, and also positive LaSota isolates (ATCC). Samples were isolated on the embryonated chicken egg and identified by HA test. The viruses were confirmed as NDV by hemagglutination inhibition assay and RT-PCR with target 687 bp. Prediction of epitope were using online software IEDB. The result of epitope B cell prediction from each isolate showing 11-12 epitope B cell candidates in protein F. Thus, it could be concluded that ND virus which isolated from Surabaya has a chance as immunogen candidate, which can be developed as vaccine candidate. Key words: Newcastle disease, Cleavage site, Fusion protein, Surabaya
Highlights
AMINO ACID ANALYSIS OF FUSION (F) GENE AND PREDICTION OF EPITOPE B-CELL NEWCASTLE DISEASE SURABAYA ISOLATE AS VACCINE CANDIDATE
Indah Laili Rahmawati et al This article is distributed under the terms of the Creative Commons Attribution License
which permits unrestricted use and redistribution provided that the original author and source are credited
Summary
Newcastle Disease merupakan penyakit endemik yang terjadi setiap tahun di Indonesia. Tempat yang paling rentan untuk terjadinya infeksi ND adalah di peternakan dan pasar unggas. Kedua tempat tersebut memiliki potensi paling tinggi untuk tertular dan merupakan sarana untuk menyebarkan virus ND (Yuliana et al, 2015). Munculnya keragaman virus dapat menyebabkan kegagalan vaksinasi sehingga diperlukan pemilihan strain vaksin yang tepat untuk menanggulangi infeksi virus ND (Miller et al, 2013). Infeksi ND pada ayam yang telah divaksinasi dapat disebabkan ayam tersebut memiliki respon antibodi yang rendah terhadap vaksin yang digunakan (Tizard, 1987; Wibawan dkk., 2003). Protein F merupakan target utama respon imun dan memiliki sifat imunogenik yang tinggi sehingga dapat dijadikan epitop imunogenik (Samal, 2012). Penelitian ini bertujuan untuk menentukan patotipe berdasarkan cleavage site gen F dari virus ND isolat Surabaya dan prediksi epitop sel-B gen fusion (F) yang memiliki sifat imunogenik yang tinggi sehingga dapat digunakan untuk pengembangan vaksin berbasis epitop di Indonesia
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