Ameliorating Effect of Standard Treatment on Cerulein-Induced Acute Pancreatitis in Rat Model

Abstract

An experiment was designed on diagnostic biomarkers and effect of standard therapy followed during acute pancreatitis in rat model. Rats were divided in three groups, A (Control), B (acute pancreatitis) and C (treatment) groups, 12 rats in each. Supra-maximal dose of cerulein (50 μg/kg b wt) was given i.p. at hourly interval for 7 h in groups B and C. Group A was treated with 0.25 ml of NSS at same interval. Treatment and sampling started after 12 h of last injection of cerulein. Group C rats were treated by antibiotic, anti-inflammatory and vit-E. Hematological and biochemical estimations were carried out at 12, 24, 60 and 156 h of last dose of cerulein. Hematological parameters found to increase significantly in group B and C than A and significant reduction noted in group C after treatment. Serum lipase and amylase were considered as accurate biomarkers and their values were found to increase several times in induction groups and became almost normal at 156 h of sampling in C group. Significant reduction in AST and ALT values noticed in treatment group. BUN and creatinine became normal in Group C at 156 and 60 h, respectively. Serum calcium became normal at 60 h. It was concluded that significant increase in serum lipase, amylase, AST, ALT, BUN, creatinine and CK-MB had diagnostic significance of acute pancreatitis and their values became normal in treatment group earlier and faster than group AP showing that standard treatment had significant role in acute pancreatitis.