Abstract
The serum concentrations of unbound LH, FSH, estradiol (E2), and free antibovine LH serum (anti-LH) were determined by RIA in hamsters destined to superovulate (∼32 ova) after a single sc injection of 0.1 ml equine anti-LH at 0900 h on day 1 of the cycle (morning of ovulation). As a control, another group of hamsters was injected with normal horse serum (NHS) at 0900 h on day 1. Animals injected with anti-LH or NHS were decapitated at various times over the next 6 days. Interfering LH antibodies were removed by column chromatography in order to determine the serum concentrations of free FSH and LH by RIA. Serum concentrations of unbound FSH were similar throughout the cycle in animals treated with anti-LH or NHS. On day 2 of the cycle (24 h after anti-LH), free LH levels were less than 6 ng/ml (the lower limit of sensitivity of the RIA on the volume of serum assayed), free anti-LH titers were extremely high, and E2 levels were less than 10 pg/ml. The absence of detectable unbound LH and normal FSH in sera...
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