Abstract
To evaluate the effects of alprostadil in an experimental model of ischemia and reperfusion injury (IRI) in rat renal tissue. Adult male Wistar rats were randomized into three groups Vehicle-treated group(Veh), Alprostadil-treated(Al), and sham(Sh) group. Veh and Al groups had suprarenal aorta occluded for 30 minutes and reperfused for 60 minutes. Saline or 20 µg/kg of Alprostadil was intravenously infused immediately before declamping. Sh group animals underwent similar procedure without aortic occlusion. Left nephrectomy and blood sampling were performed after 60 minutes of reperfusion. Renal ICAM-1 expression and histological analysis were performed to estimate inflammatory response and tissue disarrangement. Serum biochemical markers for IRI were also measured. Kruskal-Wallis test was used to assess differences between the groups. There was lower expression of ICAM-1 in groups Veh and Sh. On histologically evaluation, inflammation and necrosis in the Veh group was significantly higher (grades III/IV) than Al group (Veh>Al=Sh; p = 0.025), as well as CPK levels (Veh>Al=Sh; p = 0.03). Alprostadil attenuates the immunohistochemical and histological repercussions in the renal tissue of rats submitted to a post-ischemic reperfusion with supra-renal aortic clamping.
Highlights
Ischemia and reperfusion injury (IRI) is a syndrome characterized by an initial lack of blood flow to a region or organ, followed by reperfusion
In a significant proportion, an acidotic metabolic syndrome leading to acute tubular necrosis, cell apoptosis leading to multiorgan failure and death occurs.[2]
Tissue sections of 4 micra were mounted on slides for staining with hematoxylin and eosin or immunohistochemical staining for Intercellular adhesion molecule type 1 (ICAM-1)
Summary
Ischemia and reperfusion injury (IRI) is a syndrome characterized by an initial lack of blood flow to a region or organ, followed by reperfusion. First studies related to this syndrome are from early last century and highlighted deleterious association between extensive muscular lesions and acute renal failure, myoglobinuria, hyperpotassemia and hemodynamic symptoms.[1] With reperfusion, morphofunctional recovering occurs in the majority of cases. In a significant proportion, an acidotic metabolic syndrome leading to acute tubular necrosis, cell apoptosis leading to multiorgan failure and death occurs.[2]. This is a clinical condition frequently associated with high morbidity and mortality, especially when associated with some conditions such as aging, diabetes and renal impairment.[3] Renal insult is mostly related to the magnitude and duration of ischemia, leading to edema and tubule obstruction, mainly after reperfusion, and impaired glomerular filtration.[4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
