ALPHA-MANGOSTIN QUALITY AND QUANTITY ANALYSIS IN NAKHON SI THAMMARAT MANGOSTEEN PERICARP USING THIN-LAYER CHROMATOGRAPHY

Abstract

TLC (thin-layer chromatography) is a standard technique for simultaneously screening and monitoring chemical character in a large number of samples. The goal of this study was to explore if alpha-mangostin (a common secondary metabolite) could be detected in mangosteen pericarp phytochemical fingerprints and how much of it could be represented using a standard calibration curve. The antioxidant activity has been touted as a primary active ingredient in many commercial goods, including cosmetics and food supplements. To test for the antioxidant reaction to the DPPH onto the TLC plate, mangosteen pericarps were obtained from 12 orchards that were grown without pesticides in Cha-Uat (CU), Lan Saka (LS), and Phrom Khiri (PK) districts of Nakhon Si Thammarat. The samples were dried and powdered before being extracted with methanol using the reflux technique. After that, the TLC was utilized to determine the phytochemical fingerprint. The quality of phytochemicals from the LS orchards was found to differ from CU and PK samples, with different bands of compounds at RF 0.2, 0.25, and 0.57. The average amount of alpha-mangostin in the 12 samples was not statistically different, according to a one-way analysis of variance with a p-value of 0.05. The average range of interest (ROI) intensity area of the antioxidant was investigated using a one-way analysis of variance with a p-value of 0.05 and repeated comparisons across the sample groups by Tukeys multiple comparison test. The average antioxidant reaction between the CU and PS group and the CU and PK group was significantly different.