Abstract
This study presents a modified technique for dissociating rat exorbital lacrimal gland tissue into single cells for the in vitro study of enzyme secretion. Lacrimal gland slices were treated with collagenase and hyaluronidase followed by slight mechanical agitation and rinsing in fresh medium. Scanning electron microscopy showed that stromal elements disappeared after enzyme digestion and that isolated lacrimocytes have surface blebs, microvilli and ruffles. Lacrimocytes monitored by transmission electron microscopy showed a loss of secretion granules upon α -adrenergic stimulation. Monolayers of lacrimocytes were incubated with various selective α 1 -, α 2 - and β -adrenergic agonists and antagonists, and peroxidase as well as β -hexosaminidase release was measured over a period of 60 min. It appeared that the secretion of peroxidase only is enhanced by stimulation of α 1 -adrenoceptors.
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