Abstract
AbstractAntigen‐binding cells to sheep erythrocytes from non‐injected rabbits and from rabbits after a single injection of sheep red blood cells (SRBC) have been detected by the immunocyto‐adherence technique (rosette formation). The expression of immunoglobulin allotypic determinants on these antigen‐binding cells has been investigated by inhibition of rosette formation with anti‐allotype antisera.The results indicate that a and b locus allotypes are expressed by the great majority of rosette‐forming cells (RFC) in the lymph nodes of injected and non‐injected rabbits. Ms3 allotype is expressed by practically all RFC from non‐injected rabbits, while, after injection of SRBC, a population of RFC appears, which does not express this allotype. An antiserum to d 11 consistently failed to inhibit RFC.Pretreatment of lymphocytes from rabbits heterozygous at the b locus with antiserum to only one allelic product produced an inhibition of rosette formation which was approximately half that obtained when antiglobulin reagents to both allelic products were added. The results suggest that in heterozygous animals, the two allelic markers are expressed on two different populations of antigen‐binding cells.Electron microscopy studies on the morphology of RFC showed that the majority of the RFC were formed around small lymphocytes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
