Abstract
BackgroundStudies have demonstrated that allicin may play critical roles in the procession of ischemia–reperfusion(I/R) injury. The purpose of this study was to investigate the protective effects of allicin on renal I/R injury by attenuating oxidative stress and apoptosis.MethodsTo establish a model of renal I/R, the right kidney underwent 12 h reperfusion after 45 min ischemia, allicin was administered intraperitoneally at concentrations of 40, 50 or 60 mg/kg. NRK-52E cells were treated with allicin at concentrations of 1, 3 or 5 μM in 24 h hypoxia/ 6 h reoxygenation(H/R) treatments. Indicators of HE, oxidative stress, apoptosis were measured to evaluate the effect of aliicin on renal I/R injury.ResultsAllicin protected renal I/R injury by ameliorating histological injury and decreasing the oxidative stress in renal tissues. Meanwhile, allicin significantly downregulated the expression of Bax and caspase-3, upregulated the expression of Bcl-2 in I/R renal tissues and H/R treated NRK-52E cells.ConclusionsAllicin may exert anti-apoptotic and antioxidative effects to promote renal function recovery in I/R renal tissues and H/R treated NRK-52E cells. Taken together, allicin may be a potential novel therapy option for future renal injury protection.
Highlights
Renal ischemia–reperfusion injury (IRI) occurs after haemorrhagic shock or major cardiovascular surgery and renal transplantation, contributing to renal function impairment and other morbidities
Renal I/R resulted in significant damage to renal function, as evidenced by the dilatation and congestion of renal tubules as well as the swelling and necrosis of renal tubular epithelial cells
To evaluate the levels of oxidative stress in renal I/R, MDA levels and activity of superoxide dismutase (SOD) enzyme were determined in renal tissue
Summary
Renal ischemia–reperfusion injury (IRI) occurs after haemorrhagic shock or major cardiovascular surgery and renal transplantation, contributing to renal function impairment and other morbidities. It is the most common cause of the renal failure or hypofunction [1]. The purpose of this study was to investigate the protective effects of allicin on renal I/R injury by attenuating oxidative stress and apoptosis. Indicators of HE, oxidative stress, apoptosis were measured to evaluate the effect of aliicin on renal I/R injury. Conclusions Allicin may exert anti-apoptotic and antioxidative effects to promote renal function recovery in I/R renal tissues and H/R treated NRK-52E cells. Allicin may be a potential novel therapy option for future renal injury protection
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