Alleviation of salinity stress on germination ofLeymus chinensisseeds by plant growth regulators and nitrogenous compounds under contrasting light/dark conditions

Abstract

The effects of plant growth regulators (PGRs) and nitrogenous compounds on alleviating salinity stress on seed germination of Leymus chinensis in light (12 h light/12 h dark) and dark (24 h dark) conditions were determined in a laboratory experiment. Seed germination was compared at various combinations of salinity and germination-promoting compounds. Seed germination percentages were 57 and 74% under nonsaline conditions in light and dark, respectively, suggesting that germination was light-inhibited. Germination decreased significantly with increasing salinity level in both light and dark conditions, and the reduction was greater in light than in dark at each salinity level. Seed germination percentage decreased to 22 and 7% in light but only to 51 and 27% in dark, in 100 and 200 mM NaCl solutions respectively. The influence of PGRs and nitrogenous compounds in alleviating salinity stress varied with light and salinity condition; e.g., gibberellins (GA(4 + 7)) significantly increased germination percentages from 22%, 7% and 0.3% to 47%, 23% and 15% in light at 100, 200 and 300 mM NaCl, respectively, while they showed no effect on germination in darkness. In contrast, sodium nitroprusside and cytokinin significantly increased germination percentage in darkness at all salinity levels, but showed no effect on germination in light at 200 and 300 mM NaCl. Fluridone was very effective in alleviating salinity stress on germination in both light and dark; however, it was lethal to seedlings. Thiourea had no effect in alleviating salinity stress in either light or dark. Thus, alleviation of salinity stress on seed germination of L. chinensis by germination-promoting compounds is strongly dependent on chemicals and light conditions.