Allelopathic effects of silverleaf nightshade (Solanum elaeagnifolium Cav.) aqueous extracts on germination and early growth of wheat, broad bean and flax

Allelopathic sorghum aqueous extracts can be used as sprays against weeds of arable lands. Water-soluble allelochemicals in the aqueous extracts may also negatively affect crops. Root aqueous extracts from the South African landrace sorghum IS9456 and the Botswanan commercial variety Mahube, with high (584.69 µg mg-1 root fresh weight) and low (17.38 µg mg-1 root fresh weight) sorgoleone contents respectively, were tested on germination, radicle length, plumule length and dry weight of goosegrass [Eleusine indica (L.) Gaertn], blackjack [Bidens pilosa (L.)], maize [Zea mays (L.)], soya bean [Glycine max (L.) Merr.] and wheat [Triticum aestivum (L.)]. Factors in five glasshouse experiments were the two sorghum varieties and four root extract solutions (0%, 5%, 10% and 20%) (w/v) arranged in a randomised complete block design with six replications. There was no significant effect (P>0.05) of variety and root aqueous extract on germination, radicle length, plumule length and dry weight of maize and on germination of wheat and goosegrass. The sorghum accession IS9456 significantly (P<0.05) reduced plumule length and dry weight of wheat and goosegrass and germination, plumule length and dry weight of soya bean and blackjack compared to Mahube. Increasing strength of root aqueous extract solution significantly (P<0.001) reduced plumule length and dry weight of wheat and goosegrass as well as germination and dry weight of soya bean and blackjack. Extracts from IS9456 had greater inhibitory effects on crop and weed germination and growth compared to those from Mahube. Due to its low sorgoleone content and weak weed suppression from its root aqueous extracts, Mahube may have low potential for use in allelopathic weed control. The sorghum accession IS9456, which also produces very high sorgoleone content, may be used in integrated weed management exploiting allelopathy from both sorgoleone and water-soluble allelochemicals, although farmers will have to be careful in terms of crop rotations since the aqueous extracts also inhibit germination and growth of some crops. Field studies may be required to further confirm allelopathic effects of root aqueous extracts from IS9456. Key words: sorghum allelopathy, aqueous extracts, germination, growth, maize, soya bean, goose grass, blackjack

The allelopathic impact of aqueous extract (2.5 and 5%) and residues (2 and 4%) of five aquatic weeds namely Alternanthera philoxeroides, Alternanthera sessilis, Conyza stricta, Polygonum barbatum and Echinochloa crus-galli was investigated on the germination and early growth of wheat (Triticum aestivum). The aqueous extracts and residues of all tested weeds at different concentrations suppressed the germination/emergence percentage, mean germination/emergence time, and germination/emergence index and early seedling growth of wheat. Of the species tested, A. philoxeroides and A. sessilis showed the higher inhibition than C. stricta, P. barbatum and E. crus-galli. The results revealed that the inhibitory effect was proportional to the concentrations of the extracts and residues. Higher concentrations of extracts and residues had the stronger inhibitory effect. Aqueous extract caused more detrimental effect as compared to soil incorporated residues. Phytotoxins were identified as caffeic acid, chlorogenic acid, ferulic acid, 4-hydroxy-3methoxybenzoic acid, gallic acid, p-coumaric acid, m-coumaric acid, syringic acid and vanilic acid. It can be concluded that rice aquatic weeds contain allelopathic compounds in their tissues which may cause allelopathic effects on subsequent wheat crop in rice-wheat cropping.

Plant-growth promoting rhizobacteria (PGPR) play a vital role in soil fertility and crop production. The rhizosphere of many crop plants has been well documented by screening PGPR for their plant-growth promoting (PGP) mechanisms. However, the rhizosphere of grass species that may act as potential habitats for novel PGPR remains relatively unexplored. Ageratina adenophora is a noxious weed that has invaded more than 40 tropical and subtropical countries in Asia, Oceania, Africa, and Europe. Its presence has led to changes in plant species composition, reducing their biodiversity and destroying ecosystem function. In this study, we screened 1,200 bacterial strains isolated from the rhizosphere soil of A. adenophora in three floristic regions in Yunnan Province, China. Samples were screened for their in vitro ability for N-fixation, production of the plant growth regulator indole-3-acetic acid (IAA), and the synthesis of 1-amino-cyclopropane-1-carboxylate (ACC) deaminase, which controls the levels of ethylene in developing plant roots. We found that 144 strains showed at least one of these PGP attributes. 16S rRNA gene sequencing showed that most (62.5%) of the samples were bacteria closely related to members of the genera Pseudomonas (27 strains), Providencia (20 strains), Chryseobacterium (14 strains), Ensifer (12 strains), Enterobacter (nine strains), and Hafnia (eight strains). Their abundance and biodiversity in the soil of individual floristic regions correlate positively with the invasion history of A. adenophora. From these PGP bacterial strains, KM_A34 (Pantoea agglomerans), KM_C04 (Enterobacter asburiae), and KM_A57 (Pseudomonas putida), which had the greatest in vitro ability of N-fixation, and IAA and ACC deaminase production, respectively, were selected. The strains were evaluated for their effect on the seed germination and growth of soybean, faba bean, pea, wheat, and Chinese cabbage other than A. adenophora. Chamber experiments showed these strains significantly (P < 0.05) increased (14.2-43.4% over the controls) germination rates of the soybean, faba bean, pea, and/or Chinese cabbage seeds. They also reduced relative seed germination times (20.8-48.8% over the controls) of soy bean, faba bean and/or wheat seeds. Greenhouse pot experiments showed that they significantly (P < 0.05) promoted the aboveground and belowground height of plant foliage (12.1-23.1% and 11.5-31.4% over the controls, respectively) and/or the dry weights (16.1-33.5% and 10.6-23.4% over the controls, respectively) of the soy bean, faba bean, pea, wheat and/or Chinese cabbage. These data indicate that the rhizosphere microbiota of A. adenophora contain a PGPR pool that may be used as bioinoculants to improve the growth and productivity of these crops.

Phytochemical analysis of crop extracts and assessment of their allelopathic effect on germination and seedling growth of wild mustard (Sinapis arvensis) and faba bean (Vicia faba var. minor)

Biochar can be produced from a wide range of organic sources with varying nutrient and metal concentrations. Before making irreversible applications of biochar to soil, a preliminary ecotoxicological assessment is desirable. First, we determined the effect of biochar type and rate on early growth of wheat in a soil-less Petri dish bioassay. Second, we investigated the effect of the same biochars on seed germination and early growth of wheat in ten soils with varying texture using a glasshouse bioassay. Finally, we investigated whether these biochars had similar effects on three plant species when grown in one soil. Biochar type and application rate influenced wheat seed germination and seedling growth in a similar manner in both the soil-less Petri dish and soil-based bioassay. Germination and early root growth of mung bean and subterranean clover differed from that of wheat in response to the five biochars. We recommend use of the soil-less Petri dish bioassay as a rapid and simple preliminary test to identify potential toxicity of biochars on seed germination and early plant growth prior to biochar application to soil.

The research on carbon materials and plants primarily focuses on their use in soil, showing variable effects. However, significant gaps exist regarding their impact under stress conditions (such as drought, salinity, etc.) and their interactions with plant physiological processes. Long-term effects on plant health and ecosystems are understudied, as are their interactions with other biofertilizers like algae extracts and plant growth-promoting rhizobacteria (PGPR). The aim of the present study was to evaluate the effects of seaweed extracts and PGPR on plant growth and some physio-biochemical properties of soft wheat (Triticum aestivum L.) and faba bean (Vicia faba L.). Two concentrations of aqueous extract from two seaweeds, Fucus spiralis (F) and Ulva rigida (U), and the PGPR bacillus strain solution S48 (S48S) were used either individually or in combination. All treatments were applied as foliar sprays. The findings showed that the combined application of Fucus spiralis extract [25%] (F), Ulva rigida extract [50%] (U), along with the PGPR S48 bacillus strain solution (S48S) promoted plant growth (50.93 cm for faba bean) and root development (17.90 cm for wheat). This treatment also resulted in increased chlorophyll content (3.25 mg/g Fresh Weight (FW) in soft wheat and 3.93 mg/g FW in faba bean), proteins (14.66 µg/mg FW in soft wheat and 23.79 µg/mg FW in faba bean), amino acids (0.46 mg/g FW in soft wheat and 0.56 mg/g FW in faba bean), and total carbohydrates (529.8 µg/ml in soft wheat and 340.7 µg/ml in faba bean) as compared to controls. On the other hand, the nitrate reductase activity was enhanced by seaweed extract application in both crops. Phosphorus (P) uptake by soft wheat and faba bean was significantly improved following the application of Ulva rigida extract [50%] (U), the PGPR S48 bacillus strain solution (S48S), and the co-treatment of Fucus spiralis extract [25%] (F) with the PGPR S48 bacillus strain solution (S48S). The findings suggest that the combined application of Fucus spiralis extract (F) and Ulva rigida extract (U) with the PGPR S48 bacillus strain solution (S48S) can significantly improve plant growth and nutrient absorption in durum wheat and faba bean. This offers a sustainable alternative to chemical fertilizers, reducing pollution and promoting healthier ecosystems. Furthermore, these biological treatments enhance plant resilience to abiotic stresses, potentially increasing yields in challenging conditions. By reducing reliance on chemical fertilizers, these practices can save money for farmers and advance sustainable agriculture.

Phytotoxicity and chemical composition of essential oils from four selected Eucalyptus species in Australia were investigated. Essential oils had stronger inhibitory effects on germination and seedling growth of silverleaf nightshade (Solanum elaeagnifolium Cav.) when compared with a commercial eucalyptus oil and with 1,8-cineole. E. salubris oil had the highest inhibition index for silverleaf nightshade germination, root growth and shoot growth, while E. spathulata had the lowest inhibitory effect except root growth. Gas chromatography-mass spectrometry analysis revealed 56 compounds present in E. salubris oil, with 1,8-cineole (57.6 %), α-pinene (10.9 %) and p-cymene (8.3 %) predominant. E. dundasii oil contained 55 identified compounds with 1,8-cineole (65.5 %) and α-pinene (19.9 %) being the richest fractions. There were 56 compounds identified from E. brockwayii oil with α-pinene (31.1 %), isopentyl isovalerate (20.2 %) and 1,8-cineole (16.9 %) as the most abundant components. E. spathulata oil contained 60 compounds, predominantly 1,8-cineole (52.9 %) and α-pinene (31.0 %). Further study is required to determine the phytoxicity of the individual identified compounds on silverleaf nightshade and whether the observed phytotoxicity is attributable to a single compound or to the synergistic effects of several compounds.

ABSTRACT: We report a naturalized alien species, Solanum elaeagnifolium Cav. from Chodo Isl., Samsanmeon,Yeosushi, Jeollanam-do province. The native distribution range of the species is the southwestern US and theadjacent region of northern Mexico. The species is related to S. nigrum L., but the species can be distinguishedfrom S. nigrum by the silver green stellate trichome on the surfaces of plants, the elongated leaf shape, and thesize and color of the flowers and fruits. Solanum elaeagnifolium Cav. belongs to the Solanum subgenus Lep-tostemonum. The species is recorded on the invasive species list in the subtropical or temperate regions of manyEuropean, Mediterranean, African, South American, Asian, and Australian countries. Therefore, we also need acareful monitoring and prevention strategy for this new invasive species in Korea. Keywords: Solanum elaeagnifolium, Solanum subgenus Leptostemonum , native distribution range, invasivespecies :      #$%& (Solanum elaeagnifolium Cav.) '()* +,- . . / 012 3  4 56, 789 .- . . ! $%& :;.1 ?@A BCDE FG H , IJ, K DL , MN4 O P Q ARE ST UVW- . ./ XY Z[ Q 1&\1]3>^ , _`a ,  , __ Q bc d _M Q e1] f ghH2 iE VjW- . k  lmE . /. no2 p , q%r. st*- .

Effects of a Nematode on Biomass and Density of Silverleaf Nightshade

Faba bean (Vicia faba L.) is among the oldest crops in the world. Globally, it is third most important feed grain legume. Currently, 58 countries produce this bean on large scale. Probably faba beans are one of the best performing crops under global warming and climate change scenario because of its unique ability to excel under all most all type of climatic conditions coupled with its wide adoptability to range of soil environment. It is a good source of lysine rich protein and good source of levadopa (L-dopa), a precursor of dopamine, can be potentially used as medicine for the treatment of Parkinson’s disease. However, its production is very low due to a number of biotic and abiotic factors. Different agronomic practices also have an effect on germination, growth, yield and yield components of faba bean. Thus, the main objective of this seminar is to review the effect of seed size and sowing depth on faba bean production.

Potential risks of nicotine on the germination, growth, and nutritional properties of broad bean

Interference of silverleaf nightshade (Solanum elaeagnifolium Cav.) with Pronto spanish peanuts (Arachis hypogaea L.) was evaluated from 1981 through 1983 in a natural occurring weed population. Treatments consisted of weed-free maintenance or weed interference for 0, 4, 8, 12 weeks and for the full season. Silverleaf nightshade stems were counted as a measure of weed regrowth in treatments maintained weed free for 0, 4, and 8 weeks. Contamination of the harvested in-shell peanuts by silverleaf nightshade berries was determined by counting the number of berries passing through the peanut combine. In-shell peanut yields were reduced by an average of 17% when silverleaf nightshade was allowed to interfere with the crop for 4 weeks. Further yield reductions of 53, 66, and 66% were observed in treatments where interference occurred for 8 and 12 weeks and for the full-season, respectively. Regression analysis conducted on yield data of individual years predicts that each week of weed-free maintenance after crop emergence results in an average of 33 to 38 kg/ha yield increase above the unweeded control. Conversely, analyses of yield data averaged over all years indicated that for each week of weed interference there would be approximately a 103 kg/ha decrease in in-shell yield compared to the weed-free control. When yield data were converted to percent of yield of weed-free controls, there was no interaction among years. Regression analysis of the converted data predicts that for each week of weed-free maintenance after crop emergence there would be a 3.7% yield increase compared to the unweeded control and that for each week of weed interference there would be a corresponding yield loss of 4.5%. Silverleaf nightshade stem counts per plot were reduced an average of 18 and 36 percent for treatments maintained weed free for 4 and 8 weeks, respectively. In 1982 analysis of fruit contamination indicated a significant difference between full-season interference and weed-free maintenance for 4 or more weeks. Differences in fruit contamination between 4, 8, and 12 weeks of weed-free maintenance were not significant. In the second year no differences in fruit contamination were observed between the weedy check and the other treatments; however, fruit production after 4 weeks of weed-free maintenance was significantly higher than after 8 and 12 weeks of weed-free maintenance. Peanut quality, disregarding contamination by silverleaf nightshade berries, was not affected by weed interference.

Laboratory studies were conducted to examine the effects of different concentrations of wild barley (Hordeum spontaneum Koch.) shoot and seed extracts on germination and seedling growth of wheat (Triticum aestivum L.) and wild barley. In this study, all wild barley shoot extract concentrations (with exception of lowest concentration) significantly reduced wheat seed germination after 7 days. Shoot extract concentrations of 60 and 120 g / L significantly reduced shoot and root lengths, shoot fresh and dry weights, and, root fresh and dry weights of wheat. Seed germination of wheat was not affected by intermediate wild barley seed extracts. Considerably, some extracts of wild barley seeds stimulated the germination and growth of wheat. Wild barley shoot and seed extracts at low level stimulated the growth of its own plant, however, its germination and seedling growth were inhibited at higher shoot extract concentrations. The results of this investigation show that wild barley shoot extracts exert more allelopathic effects on germination and growth of wheat and its own plant than those of seed extracts.

Germinated grains are extensively used in weaning and geriatric foods. Germination generally improves the nutrient content and digestibility of foods. The present investigation examined the influence of germination of faba bean, soybean, rice, and wheat on the availability of iron, zinc, calcium, manganese, and copper. Germination increased the availability of iron by >fourfold from faba bean and soybean and by >twofold from rice, whereas such a beneficial influence was not seen in wheat. The availability of zinc from rice and wheat increased to an extent of 80% and 87%, respectively, as a result of germination. However, germination reduced the availability of zinc from faba bean and soybean. Germination increased the availability of calcium from faba bean, soybean, and rice. Germination did increase the availability of manganese from soybean (14.4%) and rice (43%), whereas germination did not exert any influence on availability of copper from rice and wheat; it significantly decreased (12.7% and 14.3%, respectively) the same from faba bean and soybean. Thus, germination could be an appropriate food-based strategy to gain iron and other minerals maximally from food grains.

Allelopathic chemicals in many plants can be released into the surrounding soil and may affect the development of nearby crops. Understanding allelopathic effects can be extremely beneficial for both economic and environmental reasons. The information gained from understanding allelopathy may be used in the optimization of crop rotations. Previous studies have investigated the negative allelopathic effects of Nicotiana tabacum — tobacco, on corn and other crops. Our study investigated the allelopathic impact of tobacco on seed germination of mung bean, Vigna radiata, and organic red fife wheat, Triticum aestivum. Seeds were treated with various concentrations of tobacco leaf solution to study the effects of tobacco on germination of the seeds. The effects of tobacco on the germination rate and percentage of germination were analyzed using one-way ANOVA with Tukey HSD tests. Results showed that there was a significant decrease in germination rate at high concentrations of tobacco. Our findings suggest that allelopathic chemicals released by tobacco have detrimental effects on the germination of mung bean and red fife wheat. Therefore, when forming crop rotations, it is important to take into account and understand the allelopathic effects and interactions between different species planted in the same region.