Abstract
Microorganisms used in all experiments were isolated by selective enrichment technique. MSM broth was used in enrichment technique supplemented with 1% v/v hydrocarbon substrate (4T engine oil). Bacterial inoculation of biometric flask (containing 48 ml minimal salt medium) consisted of 1ml of a 48 hours culture. Hydrocarbon substrate (4T engine oil) was then added to 1% v/v. The GC/MS analysis was performed using a MS 5973 spectrometer coupled to 9 Hewlett Packard model 6890. I4 isolate microorganisms degraded around 76% of 4T engine oil. Gas chromatography showed significant differences in the composition of hydrocarbons at the end of the experiments.
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