Abstract
Alamethicin Channels as a Signal Transduction Element in an Immobilized Single Giant Unilamellar Vesicle
Highlights
Planar bilayer membranes, especially free-standing ones, and vesicular bilayer membranes such as uni- or multilamellar liposomes, which are often functionalized with receptors, have a variety of applications in biochemical and biosensing studies.[1,2,3,4,5,6,7,8] vesicular bilayer membranes are not able to record channel currents, except for patch-clamped giant unilamellar vesicles (GUVs) of above 10 μm diameter
If channels or related compounds are reconstituted into chemically defined GUVs, they will provide a useful platform for the development of a highly sensitive molecular sensing system, because a spherical membrane has a volume of fL level and allows us to incorporate a large number of channels
For the case of suspended GUVs, the success probability of gigaseal attainment was 35% (n = 42), while that using immobilized GUVs was 55% (n = 352). These results indicate that the immobilization of GUVs is effective for increasing the probability of gigaseal achievement
Summary
Planar bilayer membranes (planar BLMs), especially free-standing ones, and vesicular bilayer membranes such as uni- or multilamellar liposomes, which are often functionalized with receptors, have a variety of applications in biochemical and biosensing studies.[1,2,3,4,5,6,7,8] vesicular bilayer membranes are not able to record channel currents, except for patch-clamped giant unilamellar vesicles (GUVs) of above 10 μm diameter. The membrane, whose magnitudes depend on the concentration of an analyte in a solution Such membrane-bound receptor approaches using peptide channels are promising for the development of highly sensitive electrochemical and optical sensing systems. The potential of alamethicin as a signal transduction element in a channel-based GUV sensor is demonstrated with anti-bovine serum albumin (BSA) antibody (anti-BSA) as a receptor and BSA as a model analyte in the membrane-bound receptor approach. The channel properties of alamethicin[20,21,22,23,24,25,26,27,28,29] in bilayer membranes have been extensively studied, but its use as a signal transduction element in a GUV-based sensor has not been reported. Immobilizing GUVs on a chemically modified solid support improves the success probability of clamping a single GUV, and can be adapted to a flow system, leading to the easiness of washing unreacted compounds, uninserting channel molecules into bilayer membranes, and exchanging buffer solutions
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
