Abstract
A minicloning technique was used to analyse quantitatively the fraction of cells incapable of division in mass populations of human glial cells at various passage levels. The percentage of non-dividers rose from 18 to 73% between passages 11 and 40 at which further subcultivation of the mass culture became impossible. The non-dividers were predominantly arrested in G 1; only a minor fraction of G 2 arrested cells was established. Although not conclusive, the data suggest that commitment to irreversible loss of division potential increases as a function of the number of completed cell cycles which a glial cell has completed.
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